Final Exam Assignment Example | Topics and Well Written Essays - 1250 words - 4

Final Exam - Assignment ExampleP3 initiates RNAIII that codes 6-hemolysin. P2 operon is 3Kb in length. It is unique when compared to P3 since it has up to quadruple open reading frames, agrA, agrB, agrC, and agrD. The agrA controls the sensory transduction while agrB is responsible for the production of histidine phosphokinase in bacteria. Due to mutations, defects may excrete in any one of four open reading frames, the resulting strains dont have P2 and P3 transcripts, beside they function arg-. These strains cannot initiate transcription from the P2 and P3. Insertion at C1Ai site in the RNARIII region in P3 results in deactivation of arg functions.2. a) -lactamase has higher activity as compared to the normal P2 and P3 promoters, and thus -lactamase can make other existing non-useful plasmids kindred p1524 unstable. Using P2 and P3 instead of -lactamase allows expression of the p15424, and this interferes with the results.b) RN6390B, arg+ produces stronger signals for alpha and beta hemolysins than does RN69 11, a null-arg strain, whose signal is extremely weak. If the researcher utilize arg+ instead, he would not obtain the same results due to interference by other unnecessary signals.c) When a B lymphocyte secretes antibodies that are directed towards a specific epitope on an antigen, we call the antibodies monoclonal. However, when a evidentiary number of antibodies are secreted that have different affinities and specificities towards different epitopes, we call them polyclonal antibodies. B- hemolysin antibody is a polyclonal antibody, has a unspecific range of affinities and specificities and thus it can detect any antigen present in its environment.d) In anti-b blot, protein A produces a stronger signal for arg-a42 and no signal in arg-a40. The signal for B-hemolysin is present only in arg-a40 and not arg-a42. These results taut that different regions of P3 codes for different chemicals.3. The author used RN7220 because it can increase the hla